In the control group, the mean ALT level was 20 ± 5 U/L, while the mean AST level was 30 ± 8 U/L. These values are within the normal range for healthy mice. In contrast, the low-dose microplastic group exhibited elevated ALT and AST levels, with mean values of 35 ± 10 U/L and 45 ± 12 U/L, respectively. The high-dose microplastic group showed an even more pronounced increase in liver enzyme levels, with a mean ALT level of 60 ± 15 U/L and a mean AST level of 80 ± 20 U/L.

According to the graph presented earlier, the EU EV market is expected to grow from 1.2 million units sold in 2022 to 5.1 million units by 2030. This represents a compound annual growth rate (CAGR) of approximately 19.8% over the 8-year period. The growth can be attributed to several factors, including increasing consumer awareness, supportive government policies, and advancements in EV technology.

To simulate real-world conditions, we employed a distributed computing environment consisting of multiple nodes, each representing a different component of the bidding system. The user interface, auction engine, and reporting and analytics components were deployed on separate nodes to ensure optimal performance and scalability. The system was configured to handle a high volume of requests, mimicking the traffic experienced by a production-grade advertising bidding platform.